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Image Search Results
Journal: Cells
Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
doi: 10.3390/cells13221833
Figure Lengend Snippet: Selection of anti-PDPN CasMab, PMab-117. ( A ) A scheme of CasMab selection from anti-PDPN mAb-producing hybridoma clones. ( B ) Flow cytometry using PMab-117 (10 μg/mL; Red line), NZ-1 (10 μg/mL; Red line), or buffer control (Black line) against LN229, LN229/PDPN, PC-10, LN319, and PDPN-knockout LN319 (BINDS-55). ( C ) Flow cytometry using PMab-117 (10 μg/mL; Red line), NZ-1 (10 μg/mL; Red line), or buffer control (Black line) against 293FT (human embryonic kidney) and PODO/TERT256 (TERT-expressed normal kidney podocyte).
Article Snippet: Immortalized normal epithelial cell lines were maintained as follows:
Techniques: Selection, Clone Assay, Flow Cytometry, Control, Knock-Out
Journal: Cells
Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
doi: 10.3390/cells13221833
Figure Lengend Snippet: Production of PMab-117-mG 2a and reactivity to cancer cells, normal kidney podocytes, and epithelial cells. ( A ) Class-switched mouse IgG 2a mAb, PMab-117-mG 2a, was generated from PMab-117 (rat IgM). ( B ) Flow cytometry using PMab-117-mG 2a (1 μg/mL; Red line), NZ-1 (1 μg/mL; Red line), or buffer control (Black line) against LN229, LN229/PDPN, PC-10, LN319, and PDPN-knockout LN319 (BINDS-55). ( C ) Flow cytometry using PMab-117-mG 2a (1 μg/mL; Red line), NZ-1 (1 μg/mL; Red line) or buffer control (Black line) against 293FT (human embryonic kidney), PODO/TERT256 (kidney podocyte), HBEC3-KT (lung bronchus epithelial cell), hTERT-HME1 (mammary gland epithelial cell), and hTCEpi (corneal epithelial cell).
Article Snippet: Immortalized normal epithelial cell lines were maintained as follows:
Techniques: Generated, Flow Cytometry, Control, Knock-Out